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4917396 1 3-biotech 50 Datum desc year 19228 https://crc1551.com/wp-content/plugins/zotpress/
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Young LN, Sherrard A, Zhou H, et al (2026) ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms. Nat Methods 23:131–142. https://doi.org/10.1038/s41592-025-02928-4
Chatterjee A, Schuler M, Braun MG, et al (2026) Regulating Promiscuous Catalysis via Substrate-Induced Transient Assembly. Angewandte Chemie International Edition 65:e202511352. https://doi.org/10.1002/anie.202511352
Zhou H, Huertas J, Maristany MJ, et al (2025) Multiscale structure of chromatin condensates explains phase separation and material properties. Science 390:eadv6588. https://doi.org/10.1126/science.adv6588
Wilken S, Abraham GR, Saleh OA (2025) Condensation and activator/repressor control of a transcription-regulated biomolecular liquid. Soft Matter 21:9008–9017. https://doi.org/10.1039/D5SM00868A
Wilken S, Abraham GR, Saleh OA (2025) Condensation and activator/repressor control of a transcription-regulated biomolecular liquid. Soft Matter 21:9008–9017. https://doi.org/10.1039/D5SM00868A
Ruan H, Dillenburg RF, Hosseini E, et al (2025) Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases. Nat Commun 16:10117. https://doi.org/10.1038/s41467-025-66327-1
Ruan H, Dillenburg RF, Hosseini E, et al (2025) Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases. Nat Commun 16:10117. https://doi.org/10.1038/s41467-025-66327-1
Ruan H, Dillenburg RF, Hosseini E, et al (2025) Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases. Nat Commun 16:10117. https://doi.org/10.1038/s41467-025-66327-1
Huang Z-F, Vrugt M te, Wittkowski R, Löwen H (2025) Anomalous grain dynamics and grain locomotion of odd crystals. Proceedings of the National Academy of Sciences 122:e2511350122. https://doi.org/10.1073/pnas.2511350122
Holl MP, Steinberg AB, te Vrugt M, Thiele U (2025) Motility-Induced Crystallization and Rotating Crystallites. Phys Rev Lett 135:158301. https://doi.org/10.1103/m3dy-53yc
Holl MP, Steinberg AB, te Vrugt M, Thiele U (2025) Motility-Induced Crystallization and Rotating Crystallites. Phys Rev Lett 135:158301. https://doi.org/10.1103/m3dy-53yc
Schumbera E, Dormann D, Walther A, Andrade-Navarro MA (2025) Computational investigation of the sequence context of arginine/glycine-rich motifs in the human proteome. BMC Genomics 26:883. https://doi.org/10.1186/s12864-025-12132-5
Dhiman S, Vleugels MEJ, Post RAJ, et al (2025) Reciprocity in dynamics of supramolecular biosystems for the clustering of ligands and receptors. Proceedings of the National Academy of Sciences 122:e2500686122. https://doi.org/10.1073/pnas.2500686122
Xie M, Chen W, Vonk-de Roy M, Walther A (2025) Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus. Nat Commun 16:8254. https://doi.org/10.1038/s41467-025-63445-8
Bell C, Chen L, Maristany MJ, et al (2025) An electrostatic repulsion model of centromere organisation
te Vrugt M (2025) Wigner Functions. Encyclopedia 5:118. https://doi.org/10.3390/encyclopedia5030118
Alberti S, Arosio P, Best RB, et al (2025) Current practices in the study of biomolecular condensates: a community comment. Nat Commun 16:7730. https://doi.org/10.1038/s41467-025-62055-8
Hudina E, Schott-Verdugo S, Junglas B, et al (2025) The bacterial ESCRT-III PspA rods thin lipid tubules and increase membrane curvature through helix α0 interactions. Proceedings of the National Academy of Sciences 122:e2506286122. https://doi.org/10.1073/pnas.2506286122
Gaurav K, Busetto V, Páez-Moscoso DJ, et al (2025) Multi-scale Simulations of MUT-16 Scaffold Protein Phase Separation and Client Recognition. Biophysical Journal. https://doi.org/10.1016/j.bpj.2025.08.001
Chen W, Dúzs B, Argudo PG, et al (2025) Ballistic diffusion fronts in biomolecular condensates. Nat Nanotechnol 20:1062–1070. https://doi.org/10.1038/s41565-025-01941-0
Chen L, Maristany MJ, Farr SE, et al (2025) Nucleosome spacing can fine-tune higher-order chromatin assembly. Nat Commun 16:6315. https://doi.org/10.1038/s41467-025-61482-x
Chen L, Maristany MJ, Farr SE, et al (2025) Nucleosome spacing can fine-tune higher-order chromatin assembly. Nat Commun 16:6315. https://doi.org/10.1038/s41467-025-61482-x
Hartmann E, Buhr J, Riedmiller K, et al (2025) KIMMDY: a biomolecular reaction emulator
Hubrich D, Valverde JA, Lee CY, et al (2025) Variant characterization in the intrinsically disordered human proteome
Xu L, Silva MJSA, Coelho JAS, et al (2025) Chemoselective dual functionalization of proteins via 1,6-addition of thiols to trifunctional N-alkylpyridinium. Nat Commun 16:5278. https://doi.org/10.1038/s41467-025-60237-y
Schäfer LV, Stelzl LS (2025) Deciphering driving forces of biomolecular phase separation from simulations. Current Opinion in Structural Biology 92:103026. https://doi.org/10.1016/j.sbi.2025.103026
Ren Y, Zhou Z, Harley I, et al (2025) Intracellular assembly of supramolecular peptide nanostructures controlled by visible light. Nat Synth 4:673–683. https://doi.org/10.1038/s44160-025-00751-5
Zippo E, Dormann D, Speck T, Stelzl LS (2025) Molecular simulations of enzymatic phosphorylation of disordered proteins and their condensates. Nat Commun 16:4649. https://doi.org/10.1038/s41467-025-59676-4
Ausserwöger H, Scrutton R, Fischer CM, et al (2025) Biomolecular condensates sustain pH gradients at equilibrium through charge neutralisation
Zhou H, Hutchings J, Shiozaki M, et al (2025) Quantitative spatial analysis of chromatin biomolecular condensates using cryoelectron tomography. Proc Natl Acad Sci U S A 122:e2426449122. https://doi.org/10.1073/pnas.2426449122
Ruan H, Lemke EA (2025) Resolving Conformational Plasticity in Mammalian Cells with High-Resolution Fluorescence Tools. Annual Review of Physical Chemistry 76:103–128. https://doi.org/10.1146/annurev-physchem-082423-030632
Strom JM, Luck K (2025) Bias in, bias out - AlphaFold-Multimer und die strukturelle Komplexität von Proteinschnittstellen. Current Opinion in Structural Biology 91:103002. https://doi.org/10.1016/j.sbi.2025.103002
Schauenburg D, Weil T (2025) Not So Bioorthogonal Chemistry. J Am Chem Soc 147:8049–8062. https://doi.org/10.1021/jacs.4c15986
Cutts EE, Tetiker D, Kim E, Aragon L (2025) Molecular mechanism of condensin I activation by KIF4A. EMBO J 44:682–704. https://doi.org/10.1038/s44318-024-00340-w
Zhou H, Huertas J, Maristany MJ, et al (2025) Multi-scale structure of chromatin condensates rationalizes phase separation and material properties
Amato M, Hwang JH, Rios MU, et al (2025) Polo-Like Kinase 1 Phosphorylation Tunes the Functional Viscoelastic Properties of the Centrosome Scaffold. Advanced Science 12:e11682. https://doi.org/10.1002/advs.202511682
Dormann D, Fröhlich R, Dias T, et al (2025) Polymers: The Hidden Heroes of Life. In: Betz UAK (ed) Science for a Better Tomorrow: Curious 2024 Insights. Springer Nature Switzerland, Cham, pp 143–157
Dormann D, Fröhlich R, Dias T, et al (2025) Polymers: The Hidden Heroes of Life. In: Betz UAK (ed) Science for a Better Tomorrow: Curious 2024 Insights. Springer Nature Switzerland, Cham, pp 143–157
Yu M, Gruzinov AYu, Ruan H, et al (2024) A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proceedings of the National Academy of Sciences 121:e2415220121. https://doi.org/10.1073/pnas.2415220121
Yu M, Gruzinov AYu, Ruan H, et al (2024) A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proceedings of the National Academy of Sciences 121:e2415220121. https://doi.org/10.1073/pnas.2415220121
Brzezinski M, Argudo PG, Scheidt T, et al (2024) Protein-Specific Crowding Accelerates Agaging in Protein Condensates. Biomacromolecules. https://doi.org/10.1021/acs.biomac.4c00609
Bhattacharjee R, Lemke EA (2024) Potential vs Challenges of Expanding the Protein Universe With Genetic Code Expansion in Eukaryotic Cells. Journal of Molecular Biology 436:168807. https://doi.org/10.1016/j.jmb.2024.168807
Ambadi Thody S, Clements HD, Baniasadi H, et al (2024) Small-molecule properties define partitioning into biomolecular condensates. Nat Chem 16:1794–1802. https://doi.org/10.1038/s41557-024-01630-w
Ambadi Thody S, Clements HD, Baniasadi H, et al (2024) Small-molecule properties define partitioning into biomolecular condensates. Nat Chem 16:1794–1802. https://doi.org/10.1038/s41557-024-01630-w
Schartel L, Jann C, Wierczeiko A, et al (2024) Selective RNA pseudouridinylation in situ by circular gRNAs in designer organelles. Nat Commun 15:9177. https://doi.org/10.1038/s41467-024-53403-1
Schartel L, Jann C, Wierczeiko A, et al (2024) Selective RNA pseudouridinylation in situ by circular gRNAs in designer organelles. Nat Commun 15:9177. https://doi.org/10.1038/s41467-024-53403-1
Schartel L, Jann C, Wierczeiko A, et al (2024) Selective RNA pseudouridinylation in situ by circular gRNAs in designer organelles. Nat Commun 15:9177. https://doi.org/10.1038/s41467-024-53403-1
Young LN, Sherrard A, Zhou H, et al (2024) ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms
Junglas B, Kartte D, Kutzner M, et al (2024) Structural basis for Vipp1 membrane binding: from loose coats and carpets to ring and rod assemblies. Nat Struct Mol Biol 1-16. https://doi.org/10.1038/s41594-024-01399-z
Changiarath A, Arya A, Xenidis VA, et al (2024) Sequence determinants of protein phase separation and recognition by protein phase-separated condensates through molecular dynamics and active learning. Faraday Discuss. https://doi.org/10.1039/d4fd00099d
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