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X-WR-CALDESC:Events for SFB 1551
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DTSTART:20240101T000000
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DTSTART;VALUE=DATE:20251027
DTEND;VALUE=DATE:20251101
DTSTAMP:20250925T084138Z
CREATED:20250925T083027Z
LAST-MODIFIED:20250925T084138Z
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SUMMARY:SFB 1551 Advanced Microscopy Techniques to study (Bio)polymer Dynamics Workshop 2025
DESCRIPTION:Content is protected.
URL:https://crc1551.com/our-event/sfb-1551-quantitative-microscopy-for-biopolymers-workshop-2025/
LOCATION:Microscopy & Histology Core Facility (MHCF) – IMB\, Ackermannweg 4\, Mainz\, 55128\, Germany
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DTSTART;TZID=UTC:20251031T103000
DTEND;TZID=UTC:20251031T113000
DTSTAMP:20251008T145514Z
CREATED:20250217T092318Z
LAST-MODIFIED:20251008T145514Z
UID:10000090-1761906600-1761910200@crc1551.com
SUMMARY:SFB 1551 Seminar Series Event - Prof. Dr. Dorothee Staiger
DESCRIPTION:Partners in time – the RNA interactome of plant RNA-binding proteins \nRNA-based regulation plays a key role in plant responses to endogenous cues and environmental stimuli. To provide insights into in vivo RNA-protein interactions shaping the transcriptome\, we improved our previous protocol for individual nucleotide resolution crosslinking and immunoprecipitation and\, in collaboration with Kathi Zarnack\, established a bioinformatics pipeline to evaluate iCLIP-seq data (plant iCLIP2). We comprehensively profiled the binding landscape of Arabidopsis thaliana glycine-rich RNA binding protein 7 implicated in different steps of RNA processing and function. In the compendium of target transcripts\, we delineated motifs preferentially associated with binding sites. Orthogonal transcriptomic data obtained from loss-of function mutants and plants overexpressing the RNA binding proteins provided insights into the mode of action how AtGRP7 affects its target transcripts. In particular\, in collaboration with Martin Crespi we pursue the idea that long noncoding RNAs interacting with AtGRP7 and the splicing regulator NSRa serve as regulators of alternative splicing. In turn\, we developed single mRNA interactome capture and identified ALBA4\, a protein interacting with m6A readers\, as an AtGRP7 interactor. Collectively\, our data begin to shed light on the organisation of networks in posttranscriptional regulation in Arabidopsis.
URL:https://crc1551.com/our-event/sfb-1551-seminar-series-event-prof-dr-dorothee-staiger/
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