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DTSTART;TZID=UTC:20251205T090000
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DTSTAMP:20251124T095029Z
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SUMMARY:SFB 1551 Seminar Series Event - Dr. Sergio Cruz-Leon
DESCRIPTION:“Bridging cellular 3D imaging with physics-based simulations for biological discovery” \nDr. Sergio Cruz-Leon (MPI Biophysics\, Frankfurt) \nSummary: \nCellular function is governed by the structure\, location\, and dynamics of its densely packed molecular machines. Cryo-electron tomography (cryoET) captures 3D snapshots of intact cells at near molecular resolution. However\, extracting reliable molecular identities and interactions from tomographic data remains challenging. \nI will show how high-confidence 3D template matching (hcTM) for cryoET [1]\, coupled with multiscaling\, enables us to model and simulate biological landscapes directly from cryoET data [1-3]. hcTM reliably localizes a wide range of macromolecular complexes in crowded cytoplasm and nuclei\, producing spatial maps that are the basis to build simulation-ready models of subcellular environments. \nUsing this framework\, we uncovered vault particles loaded with ribosomal cargo [1] and the unexpected ‘cracking’ of the nuclear pore complex (NPC) during HIV passage [2]\, and resolved the molecular architecture of human chromatin in situ [3]. We then applied it to an autophagy-related condensate directly in cells\, demonstrating that a single amino-acid substitution strengthens intermolecular interactions\, drives a liquid-to-glass mesoscale transition\, and abolishes autophagic clearance. We thereby link a molecular alteration to changes in condensate material properties and biological function. \nIntegrating in situ imaging with multiscale simulation provides a pipeline for visual proteomics and sets the stage for simulating cellular processes in their native context. \n  \nKeywords: Visual proteomics\, cryoET\, high-confidence template matching\, multi-scale simulations\, HIV\, chromatin\, biomolecular condensates. \n  \n[1] Cruz-León\, et al.\, Nat. Comm.\, 2024 \n[2] Kreysing*\, Heidari*\, Zila*\, et al.\, Cell\, 2025 \n[3] Kreysing*\, Cruz-León*\, Betz*\, et al.\, BioRxiv\, 2025
URL:https://crc1551.com/our-event/sfb-1551-seminar-series-event-dr-sergio-cruz-leon/
LOCATION:BZ2 Room 02.721 (2nd floor)\, Hans-Dieter-Hüsch-Weg 17\, Mainz\, Germany
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