SFB 1551 Seminar Series Event – Dirk Schneider & Nadja Hellmann

Isolating and characterizing proteins for in vitro condensate formation might be problematic!

Have you ever worked with isolated proteins? Most work in our group involves the isolation of (typically recombinantly produced) proteins plus their biophysical characterization. During this work, we encountered several problems associated with the insolation of tagged proteins and/or with their biophysical characterization. We have therefore decided to discuss some problems that typically occur but are often ignored “hands on”. We will focus on the isolation of tagged proteins plus their characterization via CD spectroscopy and/or dynamic light scattering. We will use examples from our own research with a condensate-forming protein to highlight some problems and pitfalls.