SFB 1551 Seminar Series Event – Dr. Martin Möckel

Methods talk by Dr. Martin Möckel, Institute of Molecular Biology (IMB)

In vitro veritas – an overview on recombinant protein expression, purification and quality control

Abstract:

Recombinant proteins are critical for basic research, for example to prove molecular mechanisms, determine protein structure and study the biophysical properties of proteins in a well-defined environment. In this talk I will give you a glimpse on the art of protein production as is done in our core facility. We will start by looking at molecular cloning techniques for expression vector generation, as well as pros and cons of various expression systems, with a focus on E. coli and the baculovirus-insect cell system. This will be followed by a more detailed explanation of various chromatography techniques, before ending the talk with a short overview of possible quality control measurements.