Congratulations to Kumar Gaurav, Lukas Stelzl and Rene Ketting from SFB1551 on their latest paper “Multi-scale Simulations of MUT-16 Scaffold Protein Phase Separation and Client Recognition,” just published in the Biophysical Journal!
Phase separation of proteins plays a critical role in cellular organisation. How phase separated protein condensates underpin biological function and how condensates achieve specificity remain elusive. We investigated the phase separation of MUT-16, a scaffold protein in Mutator foci, and its role in recruiting the client protein MUT-8, a key component in RNA silencing in C. elegans. We employed a multiscale approach that combined coarse-grained (residue-level CALVADOS2 and near-atomistic Martini3) and atomistic simulations. Simulations across different resolutions provide a consistent perspective on how MUT-16 condensates recruit MUT-8, enabling the fine-tuning of chemical details while balancing the computational cost. Both coarse-grained models (CALVADOS2 and Martini3) predicted the relative phase separation propensities of MUT-16’s disordered regions, which we confirmed through in vitro experiments. Simulations also identified key sequence features and residues driving phase separation while revealing differences in residue interaction propensities between CALVADOS2 and Martini3. Furthermore, Martini3 and 350 μs atomistic simulations on Folding@Home of MUT-8’s N-terminal prion-like domain with MUT-16 M8BR cluster highlighted the importance of cation-π interactions between Tyr residues of MUT-8 and Arg residues of MUT-16 M8BR. Lys residues were observed to be more prone to interact in Martini3. Atomistic simulations revealed that the guanidinium group of Arg also engages in sp2-π interactions and hydrogen bonds with the backbone of Tyr, possibly contributing to the greater strength of Arg–Tyr interactions compared to Lys–Tyr, where these additional favourable contacts are absent. In agreement with our simulations, in vitro co-expression pulldown experiments demonstrated a progressive loss of MUT-8 recruitment following the mutation of Arg in MUT-16 M8BR to Lys or Ala, confirming the critical role of Arg in this interaction. These findings advance our understanding of MUT-16 phase separation and subsequent MUT-8 recruitment, key processes in assembling Mutator foci that drive RNA silencing in C. elegans.
Through a collaborative effort integrating expertise from multiple integrating expertise from multiple laboratories at ImP and KOMET1 at Johannes Gutenberg University Mainz, Institute of Molecular Biology (IMB), Max Perutz Labs Vienna, University of Vienna and the Flatiron Institute, they combined multiscale simulations with biochemical validation to reveal how MUT-16 phase separation drives Mutator foci assembly and client recruitment, a molecular process underpinning small RNA biogenesis and gene regulation in C. elegans.
Congratulations to the authors on this impressive achievement!